Aim of the course: Overview of novel CRISPR-based methodologies for genome editing and derivative technologies.
Major topics: History of CRISPR; Accuracy of CRISPR/Cas9-based genome editing and High Fidelity Cas9 variants; RNA-targeting CRISPR systems; Anti-CRISPR strategies; High-throughput CRISPR screens; CRISPR-based molecular tools; Base editors; The use of CRISPR in agriculture; Human uses of genome editing and their ethics.
This course provides an in-depth exploration of revolutionary CRISPR-based genome editing technologies. Students will trace the origins of CRISPR, from the initial discovery of CRISPR-Cas9 in bacteria to its development as a versatile genome editing platform.
Examining CRISPR fundamentals, students will learn about the molecular bases of target recognition by the Cas9 endonuclease – guide RNA complex, and how this interaction can be modified to increase specificity. Students will study high-fidelity Cas9 variants and will learn about their limitations. The course also covers alternative CRISPR systems, like RNA-targeting tools, and anti-CRISPR mechanisms that inhibit CRISPR activity.
Beyond core CRISPR-Cas9, students will delve into derivative technologies. They’ll examine high-throughput CRISPR screens for systematic genetic studies, and CRISPR-based tools like base editors that enable precise single-nucleotide changes.
We will also explore the application of novel genome editing technologies, including agricultural use for crop improvement and disease resistance. Finally, students will also consider the ethical implications of human genome editing, particularly for therapeutics and germline modifications.
By the end of the course, students will have a deep understanding of the fundamental principles, recent advancements, and multifaceted applications of CRISPR-based genome editing. They will be equipped to critically evaluate the scientific, technological, and societal implications of this transformative field.
A strong (MSc-level) understanding of basic genetics and biochemistry is a preprequisite. Students are also expected to be able to read and interpret scientific papers in English.
The papers that will be discussed during classes will be provided in advance through Canvas.
Recommended reading also includes the following books:
Students will have the present and critically interpret the findings in some key papers of the CRISPR field. The instructor will oversee and guide these discussions, provide relevant feedback to the presenters, and also give more detailed background information when necessary.
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Funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Education and Culture Executive Agency (EACEA). Neither the European Union nor EACEA can be held responsible for them.
